Journal: Cell reports

Article Title: HRS mediates tumor immune evasion by regulating proteostasis-associated interferon pathway activation

doi: 10.1016/j.celrep.2023.113352

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-GAPDH (clone D16H11) , Cell Signaling Technology , Cat#5174; RRID:AB_10622025.

Techniques: Virus, Recombinant, SYBR Green Assay, Staining, shRNA, Plasmid Preparation, Software, CRISPR

Journal: Immunity

Article Title: Interferon-independent activities of mammalian STING mediate antiviral response and tumor immune evasion

doi: 10.1016/j.immuni.2020.06.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb Cell signaling Cat#5483 TBK1/NAK (D1B4) Rabbit mAb Cell signaling Cat#3504 Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb Cell signaling Cat #4947 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb Cell signaling Cat#3033 NF-κB p65 (D14E12) XP® Rabbit mAb Cell signaling Cat#8242 STING (D1V5L) Rabbit mAb (Rodent Preferred) Cell signaling Cat#50494 GAPDH (D16H11) XP® Rabbit mAb Cell signaling Cat#5174 LC3B Antibody Novus Cat#NB100–2220 PE anti-mouse CD3 Antibody Biolegend Cat#100206 FITC anti-mouse CD8a Antibody Biolegend Cat#100706 APC anti-mouse CD45 Antibody Biolegend Cat#103112 PE/Dazzle™ 594 anti-mouse IFN-γ Antibody Biolegend Cat#505845 FITC anti-mouse TNF-α Antibody Biolegend Cat#506304 PE anti-mouse Perforin Antibody Biolegend Cat#154305 APC-Granzyme B Invitrogen Cat#17-8898-80 CD3e Monoclonal Antibody (145–2C11) Invitrogen Cat#16-0031-81 CD28 Monoclonal Antibody (37.51) Invitrogen Cat#16-0281-82 Purified anti-mouse IFN-β Antibody BioLegend Cat#519202 Biotin anti-mouse IFN-β Antibody BioLegend Cat#508105 Chemicals, and Recombinant Proteins 2’3’-cGAMP Invivogen Cat#tlrl-nacga23–02 3’3’-cGAMP Invivogen Cat#tlrl-nacga 2’3’-c-di-AM(PS)2 (Rp,Rp) Invivogen Cat#tlrl-nacda2r-01 DMXAA Invivogen Cat#tlrl-dmx 9-Oxo-10(9H)-acridineacetic acid (CMA) Sigma Cat#17927 CellTrace™ CFSE Cell Proliferation Kit Invivogen Cat# {"type":"entrez-nucleotide","attrs":{"text":"C34554","term_id":"2370695","term_text":"C34554"}} C34554 Annexin V Apoptosis Detection Kit Biolegend Cat#640914 DNeasy Blood & Tissue Kits QIAGEN Cat#69506 Protease Inhibitor Cocktail Roche Cat#11873580001 Lipofectamine 2000 ThermoFisher scientific Cat#11668019 TRI Reagent Sigma Cat#T9424 Annexin V Binding Buffer Biolegend Cat#422201 RIPA Lysis Buffer Millipore Cat#20–188 ACK Lysing Buffer ThermoFisher scientific Cat#A1049201 Pierce™ BCA Protein Assay Kit ThermoFisher scientific Cat#23225 Polybrene Sigma Cat#TR-1003 DNase I Sigma Cat#11284932001 Collagenase Sigma Cat#C5138 iScript™ Reverse Transcription Supermix Bio-Rad Cat#1708840 iTaq™ Universal SYBR® Green Supermix Bio-Rad Cat#1725120 Naive CD8a+ T Cell Isolation Kit Miltenyi Biotec Cat#130-096-543 Pan T Cell Isolation Kit II Miltenyi Biotec Cat#130-095-130 Dual-Luciferase® Reporter Assay System Promega Cat#E1960 Recombinant Murine IL-2 PEPROTECH Cat#212–12 C-176 STING inhibitor BioVision Cat#B2341 Fixation/Permeabilization Solution Kit BD Cat#554714 HRP Avidin BioLegend Cat#405103 Recombinant Mouse IFN-β (ELISA Std.)

Techniques: Purification, Recombinant, Protease Inhibitor, Binding Assay, Lysis, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Cell Isolation, Reporter Assay, Avidin-Biotin Assay, Enzyme-linked Immunosorbent Assay, Staining, Software